Click the "Lamp On" icon in the instrument control window.
If using tungsten (W) lamps for visible range detection, replace them similarly when energy is low. Flow Cell Care
The Shimadzu SPD-20A is capable of supporting a wide range of advanced applications and techniques, including:
for a recurring error message?
This comprehensive operational guide serves as an optimized, real-world alternative to the standard user manual. It streamlines startup procedures, key parameters, routine maintenance, and critical troubleshooting to help your laboratory maximize system uptime and maintain flawless baseline stability. 1. Hardware Overview & Technical Specifications
The manual’s are often overlooked – but if you're integrating the SPD-20A with a non-Shimadzu system, those pinouts are gold.
: Access the "Function" menu on the front panel and scroll until the lamp hours are displayed.
as standard. This regulates the cell temperature from 5°C above ambient up to 50°C, neutralizing baseline drift caused by room temperature fluctuations. Superior Linearity : Offers a wide dynamic range with linearity up to
: Allow at least 30 minutes for the lamp and the temperature-controlled flow cell to stabilize to ensure a flat baseline. The Rockefeller University Key Technical Specifications Specification Wavelength Range 190 to 700 nm Noise Level Up to 2.5 AU Dual Wavelength Standard; allows simultaneous monitoring of two wavelengths Optimizing Performance Dual Wavelength Mode
At the end of every day, flush the cell with a miscible solvent (e.g., 50/50 Water/Methanol or Isopropyl Alcohol) to remove buffered mobile phases.
: The SPD-20A includes a temperature-controlled flow cell as standard to minimize baseline drift caused by room temperature fluctuations. Wavelength Programming
The Shimadzu SPD-20A is a dual-wavelength UV/Vis detector. Its core strengths are:
If you're working with a for HPLC, you already know it’s a reliable workhorse. But finding a clear, searchable, and complete user manual? That can be surprisingly frustrating.
Step-by-step "Purge" instructions to eliminate sawtooth or spiked baselines.